These animals went on to exhibit outward symptoms of influenza including fever, sneezing, and lethargy. Their application to influenza research began in 1933, when throat washings from human subjects were administered intranasally into ferrets. Most notably, ferrets are naturally susceptible to human influenza virus and are capable of viral transmission. The domestic ferret ( Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease. Collectively, these M αF-Ig mAb offer advancement compared to the existing portfolio of polyclonal anti-ferret Ig detection reagents and should be applicable to a wide array of immunologic assays including the identification of antibody-secreting cells by FCM. Additionally, a mAb that yielded surface staining of nearly all surface BCR positive cells ( i.e., pan ferret Ig) was generated. Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells. These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79 β + B cells. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. The domestic ferret ( Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza.
0 kommentar(er)
